Artigo
Evaluation of the methylation profile of exfoliated cell samples from patients with head and neck squamous cell carcinoma
Fecha
2014-05-01Registro en:
Head and Neck-Journal for the Sciences and Specialties of the Head and Neck. Hoboken: Wiley-Blackwell, v. 36, n. 5, p. 631-637, 2014.
1043-3074
10.1002/hed.23345
WOS:000334423500006
Autor
Bomfim Longo, Ana Luiza [UNIFESP]
Rettori, Marianna M. [UNIFESP]
Carvalho, Ana Carolina de [UNIFESP]
Kowalski, Luiz Paulo
Carvalho, Andre Lopes
Vettore, Andre Luiz [UNIFESP]
Institución
Resumen
BackgroundSilencing of tumor suppressor genes plays a vital role in head and neck carcinogenesis. the purposes of this study were to determine the methylation profile of exfoliated tumors cells collected from patients with head and neck squamous cell carcinoma (HNSCC) and to evaluate its prognostic significance.MethodsThe methylation profile and level of a 20-gene panel were evaluated by quantitative methylation-specific polymerase chain reaction (qMSP) in exfoliated tumor cell samples from 96 patients with HNSCC.ResultsCCNA1 (60.4%), DCC (54.2%), and TIMP3 (35.4%) were frequently methylated in these samples. Patients with exfoliated tumors cells positive for DCC methylation showed a trend toward a lower local recurrence-free survival.ConclusionThese findings indicate that a low invasive method could be used to access the methylation profile of exfoliated cells from patients with HNSCC. Moreover, our data provide evidence that hypermethylation of DCC could be useful as prognostic indicator for this malignancy. (c) 2013 Wiley Periodicals, Inc. Head Neck 36: 631-637, 2014