Artigo
Effects of low-intensity non-coherent light therapy on the inflammatory process in the calcaneal tendon of ovariectomized rats
Fecha
2016Registro en:
Lasers In Medical Science. London, v. 31, n. 1, p. 33-40, 2016.
0268-8921
10.1007/s10103-015-1821-7
WOS:000367695100006
Autor
Helrigle, Carla
Camilo de Carvalho, Paulo deTarso
Casalechi, Heliodora Leao
Pinto Leal-Junior, Ernesto Cesar
Cardoso Fernandes, Guilherme Henrique
Helrigel, Panmera Almeida
Rabelo, Rogerio Leao
Aleixo-Junior, Ivo de Oliveira
Aimbire, Flavio [UNIFESP]
Albertini, Regiane
Institución
Resumen
The aim of this experimental study was to investigate the effects of low-intensity light-emitting diode (LED) phototherapy on the inflammatory process in the calcaneal tendon of ovariectomized rats (OVX) through the involvement of the inflammatory mediators interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-alpha). Thirty-five female Wistar rats were divided into 4 groups: 3 groups of OVX rats totaling 30 rats (untreated OVX rats [OVX injury group], treated OVX rats [OVX LED group], and control OVX rats subgroups existed based on the sampling times, which were 3, 7, and 14 days) and 1 group of non-OVX rats (not OVX n = 5). Tendon injury was induced by trauma using a 208-g mass placed at 20 cm from the right tendon of each animal with energy of 0.70 J. The animals were treated 12 h after tendonitis with LED therapy and every 48 h thereafter until euthanasia (at 3, 7, or 14 days). The tendons were dissected and stored in liquid nitrogen at -196 A degrees C, thawed only at the time of immunoenzymatic testing (ELISA). Groups treated with LED showed a decrease in the number of pro-inflammatory cells, IL-6, and TNF-alpha (p < 0.05), and an increase in IL-10 (p < 0.05) when compared to the not OVX group (p < 0.05). It was concluded that low-intensity LED treatment using the parameters and wavelength of 945 nm in the time periods studied reduced the release of IL-6 and TNF-alpha and increased the release of IL-10, thereby improving the inflammatory response in OVX rats.