Artigo
Purification of the 43 kDa glycoprotein from exocellular components excreted by Paracoccidioides brasiliensis in liquid culture (TOM medium)
Fecha
1991-01-01Registro en:
Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 29, n. 1, p. 57-60, 1991.
0268-1218
10.1080/02681219180000101
WOS:A1991FH18100009
Autor
Puccia, Rosana [UNIFESP]
Takaoka, D. T.
Travassos, Luiz Rodolpho [UNIFESP]
Institución
Resumen
The 43 kDa glycoprotein (gp43) antigen has been purified by affinity chromatography and Sephacryl S-200 gel filtration from concentrated and dialysed culture supernatant fluids of Paracoccidioides brasiliensis grown in a tomato juice-enriched complex medium (TOM). In the TOM medium, there was rapid and extensive growth of the fungus, with an abundant production of gp43 in relation to other exocellularly accumulated components. The purified gp43 can be used for immunochemical and structure determination studies.