Artigo
A TSH-CREB1-microRNA Loop Is Required for Thyroid Cell Growth
Fecha
2011-10-01Registro en:
Molecular Endocrinology. Chevy Chase: Endocrine Soc, v. 25, n. 10, p. 1819-1830, 2011.
0888-8809
10.1210/me.2011-0014
WOS:000295322700012
Autor
Leone, Vincenza
D'Angelo, Daniela
Ferraro, Angelo
Pallante, Pierlorenzo
Rubio, Ileana [UNIFESP]
Santoro, Massimo
Croce, Carlo Maria
Fusco, Alfredo
Institución
Resumen
MicroRNA (miRNA or miR) are an important class of regulators that participate in such biological functions as development, cell proliferation, differentiation, and apoptosis. the aim of this study was to elucidate the role of miRNA in cell proliferation using a unique cell system, namely thyroid cells that require thyrotropin for their growth. Here, we report the identification of a set of five specific miRNA (miR-1, miR-28-A, miR-290-5p, miR-296-3p, and miR-297a), whose down-regulation by thyrotropin is required for thyroid cell growth. in fact, overexpression of these miRNA negatively affects cell growth. We show that three of these miRNA target cAMP-responsive element binding protein (CREB)1, a thyrotropin-activated transcription factor, and that CREB1 binds the regulatory regions of the down-regulated miRNA. Hence, these data indicate that a synergistic loop involving thyrotropin, CREB1, and miRNA is required for thyroid cell proliferation. (Molecular Endocrinology 25: 1819-1830, 2011)