Distinct effects of acute and chronic sleep loss on DNA damage in rats

dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.creatorAndersen, Monica Levy [UNIFESP]
dc.creatorRibeiro, Daniel Araki [UNIFESP]
dc.creatorBergamaschi, Cassia Toledo [UNIFESP]
dc.creatorAlvarenga, Tathiana Aparecida [UNIFESP]
dc.creatorSilva, Andressa [UNIFESP]
dc.creatorZager, Adriano [UNIFESP]
dc.creatorCampos, Ruy Ribeiro [UNIFESP]
dc.creatorTufik, Sergio [UNIFESP]
dc.date.accessioned2016-01-24T13:52:27Z
dc.date.accessioned2022-10-07T21:04:22Z
dc.date.available2016-01-24T13:52:27Z
dc.date.available2022-10-07T21:04:22Z
dc.date.created2016-01-24T13:52:27Z
dc.date.issued2009-04-30
dc.identifierProgress in Neuro-psychopharmacology & Biological Psychiatry. Oxford: Pergamon-Elsevier B.V., v. 33, n. 3, p. 562-567, 2009.
dc.identifier0278-5846
dc.identifierhttps://repositorio.unifesp.br/handle/11600/31460
dc.identifier10.1016/j.pnpbp.2009.02.014
dc.identifierWOS:000265470500025
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/4026589
dc.description.abstractThe aim of this investigation was to evaluate genetic damage induced in male rats by experimental sleep loss for short-term (24 and 96 h) and long-term (21 days) intervals, as well as their respective recovery periods in peripheral blood, brain, liver and heart tissue by the single cell gel (comet) assay. Rats were paradoxically deprived of sleep (PSD) by the platform technique for 24 or 96 h, or chronically sleep-restricted (SR) for 21 days. We also sought to verify the time course of their recovery after 24 h of rebound sleep. the results showed DNA damage in blood cells of rats submitted to PSD for 96 h. Brain tissue showed extensive genotoxic damage in PSD rats (both 24 and 96 h), though the effect was more pronounced in the 96 h group. Rats allowed to recover from the PSD-96 h and SR-21 days treatments showed DNA damage as compared to negative controls. Liver and heart did not display any genotoxicity activity. Corticosterone concentrations were increased after PSD (24 and 96 h) relative to control rats, whereas these levels were unaffected in the SR group. Collectively, these findings reveal that sleep loss was able to induce genetic damage in blood and brain cells, especially following acute exposure. Since DNA damage is an important step in events leading to genomic instability, this study represents a relevant contribution to the understanding of the potential health risks associated with sleep deprivation. (C) 2009 Elsevier Inc. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationProgress in Neuro-psychopharmacology & Biological Psychiatry
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.rightsAcesso restrito
dc.subjectChronic sleep deprivation
dc.subjectCorticosterone
dc.subjectDNA damage
dc.subjectRats
dc.subjectRebound
dc.subjectSingle cell gel (comet) assay
dc.titleDistinct effects of acute and chronic sleep loss on DNA damage in rats
dc.typeResenha


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