Artigo
Phytochemical analysis of hydroethanolic extract of turnera diffusa willd and evaluation of its effects on astrocyte cell death
Análise fitoquímica do extrato hidroetanólico de Turnera diffusa Willd e avaliação de seus efeitos na morte de astrócitos
Fecha
2016Registro en:
Einstein-Sao Paulo. Sao paulo sp, v. 14, n. 1, p. 56-63, 2016.
1679-4508
S1679-45082016000100056.pdf
S1679-45082016000100056
10.1590/S1679-45082016AO3386
WOS:000385715800012
Autor
Bezerra, Andreia Gomes [UNIFESP]
Negri, Giuseppina [UNIFESP]
Duarte-Almeida, Joaquim Mauricio
Smaili, Soraya Soubhi [UNIFESP]
Carlini, Elisaldo Araujo [UNIFESP]
Institución
Resumen
Objective: To evaluate the phytochemical composition of hydroethanolic extracts from powdered aerial parts of Turnera diffusa Willd (Turneraceae T. diffusa), as well as its toxicity in astrocytes. Methods: Chemical analyses of hydroethanolic extract from powdered aerial parts of T. diffusa were carried out using HPLC-DAD-ESI-MS/MS. In vitro assays using astrocytes culture were performed to evaluate cell death. Results: Flavone-C, O-diglycosides, such as, luteolin-8-C-[6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside, apigenin-8-C-[ 6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside and apigenin-7-O-6"-p-coumaroylglucoside were the main compounds found in this hydroethanolic extract. Concentration time-effect demonstrated the toxicity of this extract at a concentration of 1,000 mu g/mL in astrocyte culture, after 6 and 24 hours of incubation. Conclusion: In phytochemical analyses, important antioxidants (mainly flavonoids) were observed. T. diffusa extracts presented cytotoxic effect in high concentrations, leading to increased cell death in astrocyte culture.