Artigo
Interaction of an esophageal MEG protein from schistosomes with a human S100 protein involved in inflammatory response
Fecha
2017Registro en:
Biochimica Et Biophysica Acta-General Subjects. Amsterdam, v. 1861, n. 1, p. 3490-3497, 2017.
0304-4165
10.1016/j.bbagen.2016.09.015
WOS:000390736300053
Autor
Orcia, Debora
Zeraik, Ana Eliza
Lopes, Jose L. S.
Macedo, Joci N. A.
dos Santos, Clarissa Romano
Oliveira, Katia Cristina [UNIFESP]
Anderson, Leticia
Wallace, B. A.
Verjovski-Almeida, Sergio
Araujo, Ana P. U.
DeMarco, Ricardo
Institución
Resumen
Background: The Micro-Exon Gene-14 (MEG-14) displays a remarkable structure that allows the generation of antigenic variation in Schistosomes. Previous studies showed that the soluble portion of the MEG-14 protein displays features of an intrinsically disordered protein and is expressed exclusively in the parasite esophageal gland. These features indicated a potential for interaction with host proteins present in the plasma and cells from ingested blood. Methods: A yeast two-hybrid experiment using as bait the soluble domain of Schistosoma mansoni MEG-14 (sMEG-14) against a human leukocyte cDNA library was performed. Pull-down and surface plasmon resonance (SPR) experiments were used to validate the interaction between sMEG-14 and human S100A9. Synchrotron radiation circular dichroism (SRCD) were used to detect structural changes upon interaction between sMEG-14 and human S100A9. Feeding of live parasites with S100A9 attached to a fluorophore allowed the tracking of the fate of this protein in the parasite digestive system. Results: S100A9 interacted with sMEG-14 consistently in yeast two-hybrid assay, pull-down and SPR experiments. SRCD suggested that MEG-14 acquired a more regular structure as a result of the interaction with S100A9. Accumulation of recombinant S100A9 in the parasite's esophageal gland, when ingested by live worms suggests that such interaction may occur in vivo. Conclusion: S100A9, a protein previously described to be involved in modulation of inflammatory response, was found to interact with sMEG-14. General significance: Our results allow proposing a mechanism involving MEG-14 for the parasite to block inflammatory signaling, which would occur upon release of S100A9 when ingested blood cells are lysed. (C) 2016 Elsevier B.V. All rights reserved.
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