Artigo
Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
Fecha
2010-06-01Registro en:
Future Microbiology. London: Future Medicine Ltd, v. 5, n. 6, p. 971-980, 2010.
1746-0913
10.2217/FMB.10.49
WOS:000278582500017
Autor
Leao, Sylvia Cardoso [UNIFESP]
Viana-Niero, Cristina
Matsumoto, Cristianne Kayoko
Batista Lima, Karla Valeria
Lopes, Maria Luiza
Palaci, Moises
Hadad, David Jamil
Vinhas, Solange
Duarte, Rafael Silva
Silva Lourenco, Maria Cristina
Kipnis, Andre
das Neves, Zilah Candida
Alcantara Gabardo, Betina Mendez
Ribeiro, Marta Osorio
Baethgen, Ludmila
Assis, Denise Brandao de
Madalosso, Geraldine
Chimara, Erica
Dalcolmo, Margareth Pretti
Institución
Resumen
Aim: Our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in Brazil, were caused by a single mycobacterial strain. Materials & methods: Available information from 929 surgical patients was obtained from local health authorities. A total of 152 isolates from surgical patients were identified by PCR restriction enzyme analysis of the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene. Isolates were typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes. Dral and Asel. A total of 15 isolates not related to surgical cases were analyzed for comparison. Results: All isolates were identified as Mycobacterium abscessus ssp. massiliense. Isolates from surgical patients and one sputum isolate grouped in a single PFGE cluster, composed of two closely related patterns, with one band difference. A total of 14 other isolates unrelated to surgical cases showed distinctive PFGE patterns. Conclusion: A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.