A new method paper electrophoresis, involving the use of a ionophoretic technique is described for the study of Be(II) and Co(II) biologically important binary complexes with norvaline in solution. The stability constants of ML and ML2 complexes of Be(II) - norvaline and Co(II) - norvaline systems have been found to be (7.19 ± 0.02, 5.93 ± 0.09) and (4.61 ± 0.12, 2.83 ± 0.05) (logarithm stability constant values), respectively at ionic strength of 0.1 mol L-1 and atemperature of 35°C.