Artigo de Periódico
IgG anti-Ige autoantibodies in visceral leishmaniasis
Fecha
2002-01Registro en:
0074-0276
v. 97, n. 1
Autor
Atta, Ajax Mercês
Atta, Maria Luiza Brito de Sousa
D'Oliveira Junior, Argemiro
Almeida, Roque Pacheco de
Araujo, Maria Ilma Andrade Santos
Carvalho Filho, Edgar Marcelino de
Atta, Ajax Mercês
Atta, Maria Luiza Brito de Sousa
D'Oliveira Junior, Argemiro
Almeida, Roque Pacheco de
Araujo, Maria Ilma Andrade Santos
Carvalho Filho, Edgar Marcelino de
Institución
Resumen
Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa e-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.