Artigo Publicado em Periódico
Effect of organic solvent and the lipid content on functionality of bovine lung protein isolates
Fecha
1993Registro en:
0308-8146
v. 48, n. 3
Autor
Arêas, José Alfredo Gomes
Alcocer, Marcos J. C.
Mota, Eleonor Maria de Almeida
Arêas, José Alfredo Gomes
Alcocer, Marcos J. C.
Mota, Eleonor Maria de Almeida
Institución
Resumen
Protein was isolated from bovine lung and extracted with organic solvents of
varying polarities. Lipid represented 20% by weight of the total isolate, and the
amount extracted depended on the polarity of the organic solvents employed.
Phospholipids, cholesterol and free fatty acids were the lipid classes detected,
phospholipids being the major component left after solvent extraction. Water
absorption characteristics, solubility of the protein and superficial hydrophobicity
were related to the amount of lipids left after solvent extraction. The results
indicated that the lipids rather than the organic solvents used for lipid removal
affected protein characteristics. Phospholipid-protein interactions seem to induce,
on the protein, greater amounts of ordered conformation.