Artigo de Periódico
Cytoprotective Effect of Valeriana officinalis Extract on an In Vitro Experimental Model of Parkinson Disease
Fecha
2009Registro en:
0364-3190
v. 34, n. 2
Autor
Oliveria, Diêgo Madureira de
Barreto, George Emílio Sampaio
Andrade, Deyse Valverde Gomes de
Saraceno, Ezequiel
Aon Bertolino, Laura
Capani, Francisco
El-Bachá, Ramon dos Santos
Giraldez, Lisandro Diego
Oliveria, Diêgo Madureira de
Barreto, George Emílio Sampaio
Andrade, Deyse Valverde Gomes de
Saraceno, Ezequiel
Aon Bertolino, Laura
Capani, Francisco
El-Bachá, Ramon dos Santos
Giraldez, Lisandro Diego
Institución
Resumen
Parkinson0s disease (PD) is one of the most
important neurodegenerative worldwide disorders. The potential cytoprotective effects of aqueous extract of
Valeriana officinalis on rotenone-induced apoptosis in human neuroblastoma SH-SY5Y cells were demonstrated.
The cytotoxicity, cell viability and analysis of cellular morphology were performed by MTT-tetrazole (3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
assay and phase contrast microscopy, respectively. Significant
changes in the cellular morphology, and
condensation of the cell body could be observed when cells were treated with 300 nM rotenone for 48 h. Three different
concentrations of Valeriana officinalis extract were used (0.049, 0.098 and 0.195 mg/mL). These extracts brought about an increase of 7.0 ± 1.3%, 14.5 ± 1.3% and
14.5 ± 3.2% in cell viability. Our results indicated that neuroprotector action of the Valeriana officinalis extract
provides support for later studies as they help understanding this drug for the development of cytoprotective
various therapies in PD.