Artigo de Periódico
Schistosoma mansoni antigens modulate the allergic response in a murine model of ovalbumin-induced airway inflammation
Fecha
2010Registro en:
0009-9104
v. 160, n. 2
Autor
Cardoso, Luciana Santos
Oliveira, S. C.
Góes, A. M.
Oliveira, R. R.
Pacífico, L. G.
Marinho, F. V.
Fonseca, C. T.
Cardoso, F. C.
Carvalho Filho, Edgar Marcelino de
Araujo, Maria Ilma Andrade Santos
Cardoso, Luciana Santos
Oliveira, S. C.
Góes, A. M.
Oliveira, R. R.
Pacífico, L. G.
Marinho, F. V.
Fonseca, C. T.
Cardoso, F. C.
Carvalho Filho, Edgar Marcelino de
Araujo, Maria Ilma Andrade Santos
Institución
Resumen
Schistosoma mansoni infection has been associated with protection against
allergies. The mechanisms underlying this association may involve regulatory
cells and cytokines. We evaluated the immune response induced by the S.mansoni antigens Sm22·6, PIII and Sm29 in a murine model of ovalbumin(OVA)-induced airway inflammation. BALB/c mice were sensitized with subcutaneously injected OVA-alum and challenged with aerolized OVA. Mice
were given three doses of the different S. mansoni antigens. Lung histopathology,
cellularity of bronchoalveolar lavage (BAL) and eosinophil peroxidase
activity in lung were evaluated. Immunoglobulin (Ig)E levels in serum and
cytokines in BAL were also measured. Additionally, we evaluated the frequency
of CD4+forkhead box P3 (FoxP3)+ T cells in cultures stimulated with
OVA and the expression of interleukin (IL)-10 by these cells. The number of
total cells and eosinophils in BAL and the levels of OVA-specific IgE were
reduced in the immunized mice. Also, the levels of IL-4 and IL-5 in the BAL of
mice immunized with PIII and Sm22·6 were decreased, while the levels of
IL-10 were higher in mice immunized with Sm22·6 compared to the nonimmunized
mice. The frequency of CD4+FoxP3+ T cells was higher in the
groups of mice who received Sm22·6, Sm29 and PIII, being the expression of
IL-10 by these cells only higher in mice immunized with Sm22·6. We concluded
that the S. mansoni antigens used in this study are able to downmodulate
allergic inflammatory mediators in a murine model of airway inflammation and that the CD4+FoxP3+ T cells, even in the absence of IL-10 expression, might play an important role in this process.