Artigo de Periódico
Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
Fecha
2011Registro en:
1873-2542
v. 152.
Autor
Timenetsky, Jorge
Marques, Lucas Miranda
Buzinhani, Melissa
Guimarães, Ana Marcia de Sá
Oliveira, Rosângela Claret de
Yamaguti, Maurício
Marques, R. C. P.
Farias, S. T.
Lopes Neto, R.
Timenetsky, Jorge
Marques, Lucas Miranda
Buzinhani, Melissa
Guimarães, Ana Marcia de Sá
Oliveira, Rosângela Claret de
Yamaguti, Maurício
Marques, R. C. P.
Farias, S. T.
Lopes Neto, R.
Institución
Resumen
Ureaplasma diversum infection in bulls may result in seminal vesiculitis, balanoposthitis and alterations in spermatozoids. In cows, it can cause placentitis, fetal alveolitis, abortion
and the birth of weak calves. U. diversum ATCC 49782 (serogroups A), ATCC 49783
(serogroup C) and 34 field isolates were used for this study. These microorganisms were
submitted to Polymerase Chain Reaction for 16S gene sequence determination using Taq
High Fidelity and the products were purified and bi-directionally sequenced. Using the
sequence obtained, a fragment containing four hypervariable regions was selected and
nucleotide polymorphisms were identified based on their position within the 16S rRNA
gene. Forty-four single nucleotide polymorphisms (SNP) were detected. The genotypic variability of the 16S rRNA gene of U. diversum isolates shows that the taxonomy
classification of these organisms is likely much more complex than previously described
and that 16S rRNA gene sequencing may be used to suggest an epidemiologic pattern of
different origin strains.