Artigo de Periódico
Cytokine Profile and Immunomodulation in Asymptomatic Human T-Lymphotropic Virus Type 1-Infected Blood Donors
Fecha
2001Registro en:
1077-945
27(1)
Autor
Carvalho Filho, Edgar Marcelino de
Porto, Maria Aurélia da Fonseca
Bacellar, Maria Olívia Amado Ramos
Braga, Silvane
Castro, Bernardo Galvão
Neva, Franklin A.
Carvalho Filho, Edgar Marcelino de
Porto, Maria Aurélia da Fonseca
Bacellar, Maria Olívia Amado Ramos
Braga, Silvane
Castro, Bernardo Galvão
Neva, Franklin A.
Institución
Resumen
The modulation of the immune response has been used as therapy for clinical disorders
associated with human T-lymphotropic virus type 1 (HTLV-1) infection. In this study, the
cytokine profile was evaluated in 26 asymptomatic HTLV-1 blood donors. Additionally, both the cell responsible for producing interferon-γ (IFN-γ) and the role of exogenous interleukin (IL)-10 in downregulating IFN-γ production were studied. Cytokine levels were determined in supernatants of unstimulated lymphocyte cultures by enzyme-linked immunosorbent assay. The levels of IFN-γ, tumor necrosis factor-α, IL-5, and IL-10 were higher in supernatants of the lymphocyte cultures taken from HTLV-1-infected donors than
in those taken from healthy subjects. Although depletion of CD8+ T cells and natural killer cells did not affect IFN-γ production, depletion of CD4+ T cells significantly decreased IFN-γ production. Furthermore, at a concentration of 2 ng/ml, IL-10 had only a minimum effect on IFN-γ producti on, although at high concentrations (100 ng/ml), IL-10 decreased IFN-γ production by 50% in HTLV-1-infected individuals. These data indicate that both T
helper 1 and T helper 2 cytokines are elevated in HTLV-1 infection and that IL-10 in high concentrations modulates IFN-γ production in these patients.