doctoralThesis
Análise imuno-histoquímica de proteínas relacionadas às respostas Th1, Th2 e Th17 na doença periodontal
Fecha
2011-02-17Registro en:
SANTOS, Bruna Rafaela Martins dos. Análise imuno-histoquímica de proteínas relacionadas às respostas Th1, Th2 e Th17 na doença periodontal. 2011. 159 f. Tese (Doutorado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2011.
Autor
Santos, Bruna Rafaela Martins dos
Resumen
Periodontal disease is an inflammatory condition of infectious nature
characterized by destruction of protecting and supporting dental tissues. It happens as a
response produced by the host when attacked by microorganisms. Several factors are
involved in this process. Among them, cytokines are key regulatory molecules in this
immune response, playing a role either protective and/or destructive in lesion
progression. Thus, this study investigated the immunohistochemical expression of IFN-
, GATA-3, IL-17, IL-23, IL-6 and TGF- in gingival tissues of humans, in an attempt
to gain a better understanding of the participation of Th1, Th2 and Th17 immune
responses in the development of periodontal disease processes. To this end, eighty-two
samples of gingival tissues were divided into three groups: Group 1 = 15 (samples of
healthy gum tissue as controls), Group 2 = 36 (samples with chronic gingivitis) and
Group 3 = 31 (samples with chronic periodontitis). All cases were submitted to
morphological analysis from sections stained with hematoxylin and eosin and then
subjected to staining by immunohistochemistry using the streptavidin-biotin method.
Results showed positive labeling for all proteins. Nonetheless, we observed a greater
expression of Th1 cytokines and Th17 cells in group 3. We found statistically
significant difference between TGF- expression and the clinical condition of the
samples (p=0.02). We conclude that Th1 and Th17 responses may act synergistically in
the destructive process of periodontal tissue, overlapping the Th2 response that was also
present in these tissues