masterThesis
Avaliação dos parâmetros hematológicos e imunofenotípicos de pacientes com doenças linfoproliferativas crônicas no Rio Grande do Norte
Date
2014-02-26Registration in:
PAIVA, Aldair de Sousa. Avaliação dos parâmetros hematológicos e imunofenotípicos de
pacientes com doenças linfoproliferativas crônicas no Rio Grande
do Norte. 2014. 90 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal do Rio Grande do Norte, Natal, 2014.
Author
Paiva, Aldair de Sousa
Institutions
Abstract
Chronic lymphoproliferative disorders (DLPC) are lymphoid system diseases
characterized by the abnormal proliferation of mature lymphocytes that affect B
cells, T lymphocytes and NK cells. The aim of the study was to demonstrate the
relevance of immunophenotyping by flow cytometry in patients with prolonged
lymphocytosis and / or cytomorphological changes compatible with
lymphoproliferative diseases. In this study 460 patients (244 men and 216 women)
with DLPC were evaluated. Were analyzed by flow cytometry with a panel of
monoclonal antibodies consisting of CD3, CD4, CD5, CD8, CD10, CD19, CD22,
CD23, CD25, CD38, CD45, CD16/CD56, and HLADR heavy and light chains of
immunoglobulins. It also examines information regarding age, gender of patients
and laboratory data as leucocytes, cytomorphological analysis, platelet count and
hemoglobin determination. The results showed 398 cases of chronic
lymphoproliferative disorders and 62 of DLPC B cell lymphoproliferative diseases T.
B showed the following distribution : 253 cases of chronic lymphocytic leukemia
(CLL), 42 cases of multiple myeloma ( MM ), 37 cases of lymphoma non - Hodgkin
lymphoma in leukemic phase (NHL) , 17 cases of pro- B lymphocytic leukemia ( B -PLL), 15 cases of mantle cell lymphoma (MCL ), 12 cases of plasma cell leukemia (
PCL), 9 cases of lymphoma Burkitt (Linf B), 8 cases of leukemia villous cells ( LCV),
3 cases of splenic lymphoma with villous cells (LECV), a case of follicular lymphoma
(LF) and a Waldenströn macroglobulinemia ( MW). The diseases source NK / T
were 23 cases of peripheral T cell lymphoma (LCTP), 14 cases of T prolymphocytic leukemia (T -PLL), 10 cases of leukemia T of large granular
lymphocytes (LGL -T) 9 cases of leukemia cells of adult T (LCTA), 5 cases of
Sezary syndrome (SS) and a case of large granular NK leukemia (LGL -NK)
lymphocytes. In conclusion, the combined use of the monoclonal antibody panel
careful cytomorphological analysis was shown to be essential in immune diagnosis
and classification of chronic lymphoproliferative disorders. This study was approved
by the IRB - HUOL under number 356 / 09