masterThesis
Associação do receptor toll-like 2 com o estado pró-inflamatório do diabetes tipo 1
Fecha
2009-03-30Registro en:
URURAHY, Marcela Abbott Galvão. Associação do receptor toll-like 2 com o estado pró-inflamatório do diabetes tipo 1. 2009. 114 f. Dissertação (Mestrado em Bioanálises e Medicamentos) - Universidade Federal do Rio Grande do Norte, Natal, 2009.
Autor
Ururahy, Marcela Abbott Galvão
Resumen
Inflammation has been pointed out as an important factor in development of chronic
diseases, as diabetes. Hyperglycemia condition would be responsible by toll-like
receptors, TLR2 and TLR4, and, consequently by local and systemic inflammation
induction. Thus, the objective of present study was to evaluate type 1 Diabetes mellitus
(T1DM) pro-inflammatory state through mRNA expression of TLRs 2 and 4 and proinflammatory
cytokines IL-1β, IL-6 and TNF-α correlating to diabetic nephropathy. In
order to achieve this objective, 76 T1DM patients and 100 normoglycemic (NG)
subjects aged between 6 and 20 years were evaluated. T1DM subjects were evaluated as
a total group DM1, and considering glycemic control (good glycemic control
DM1G, and poor glycemic control DM1P) and considering time of diagnosis (before
achieving 5 years of diagnosis DM1< 5yrs, and after achieving 5 years of diagnosis
DM1 <5yrs). Metabolic control was evaluated by glucose and glycated hemoglobin
concentrations; to assess renal function serum urea, creatinine, albumin, total protein
and urinary albumin-to-creatinine ratio were determined and to evaluate hepatic
function, AST and ALT serum activities were measured. Pro-inflammatory status was
assessed by mRNA expression of TLRs 2 and 4 and the inflammatory cytokines IL-1β,
IL-6 and TNF-α. Except for DM1G group (18.4%), DM1NC patients (81.6%) showed a
poor glycemic control, with glycated hemoglobin (11,2%) and serum glucose (225,5
md/dL) concentrations significantly increased in relation to NG group (glucose:
76,5mg/dL and glycated hemoglobin: 6,9%). Significantly enhanced values of urea
(20%) and ACR (20,8%) and diminished concentrations of albumin (5,7%) and total
protein (13,6%) were found in T1DM patients, mainly associated to a poor glycemic
control (DM1P increased values of urea: 20% and ACR:49%, and diminished of
albumin: 13,6% and total protein:13,6%) and longer disease duration (DM1 <5yrs -
increased values of urea: 20% and ACR:20,8%, and diminished of albumin: 14,3% and
total protein:13,6%). As regarding pro-inflammatory status evaluation, significantly
increased mRNA expressions were presented for TLR2 (37,5%), IL-1β (43%), IL-6
(44,4%) and TNF-α (15,6%) in T1DM patients in comparison to NG, mainly associated
to DM1P (poor glycemic control TLR2: 82%, IL-1β: 36,8% increase) and DM1 <5yrs
(longer time of diagnosis TLR2: 85,4%, IL-1β: 46,5% increased) groups. Results
support the existence of an inflammatory state mediated by an increased expression of
TLR2 and pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in T1DM