bachelorThesis
Ação antibacteriana do extrato pirolenhoso de Eucalyptus urograndis em gel e sólido frente à Staphylococcus aureus e Pseudomonas aeruginosa
Fecha
2022-02-02Registro en:
SILVA, Kamilla Crysllayne Alves da. Ação antibacteriana do extrato pirolenhoso de Eucalyptus urograndis em gel e sólido frente à Staphylococcus aureus e Pseudomonas aeruginosa. 2022. 46f. Trabalho de Conclusão de Curso (Graduação em Engenharia Florestal), Unidade Acadêmica Especializada em Ciências Agrárias, Universidade Federal do Rio Grande do Norte, Macaíba, 2022.
Autor
Silva, Kamilla Crysllayne Alves da
Resumen
Bacterial resistance is a current problem caused by inadequate antibiotic consumption. This
resistance is acquired through the modification mechanisms that alter the genes of these
microorganisms making them stronger. Among these resistant bacteria you can mention
Staphylococcus aureus and Pseudomonas aeruginosa. The search for alternative substances
and mainly as a plant source for the production of new drugs is a possibility. The
pirolenhosextract (EP), a product of a timber source, because it has compounds in its
structure that are capable of inhibiting the growth of bacteria, thus emerging as an option for
this purpose. However, its liquid form can cause problems, when marketed, due to difficulties
in transporting products of this category. Maltodextrin for having the ability to change the
texture of solutes is adequate to modify the physical state of the pyrolenous extract. Periods
and temperatures were tested to adjust which best condition the extract corresponds to change
from state to gel and solid. Standard AATCC strains of sthapylococcus aureus and
Pseudomonas aeruginosa were used to determine the Minimum Inibitory Concentration and
Minimum Bactericidal Concentration through the well microdilution method. The
concentrations of pirolenhosextract in gel were 15, 5, 0.5 and 0.25% and solid
pirolenhosextract 60, 50, 40, 30, 20, 18, 16, 10 and 1 mg/mL, where they were used to
determine the MIC in front of the bacteria in this study. For the indication of MBC, the
treatment was manipulated, which responded as minimum inhibition and a higher
concentration, where aliquots of each species were removed and arranged in petri dish. For
the production of pirolenhosextract in gel, it was necessary 1200 g of EP, 120 g of
maltodextrin and taken to the greenhouse at 60 °C for 20 h, for the solid state this
combination was in an oven at 60 °C for 50h. The MIC of the pirolenhosextract in gel was
5% and 1% and the CBM of 5% and 5% for S. aureus and P. aeruginosa, respectively. For the
pirolenhosextract in solid, S. aureus had MIC of 10 mg/mL and CBM of 26 mg/mL, while P.
aeruginosa presented MIC of 10 mg/mL and CBM of 16 mg/mL. The results indicate that the
pirolenhosextract has bactericidal action and its change in physical state does not alter its
antimicrobial capacity.