Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets

dc.creatorSilva, Fatima C. B. L.
dc.creatorAlcazar, Alonso
dc.creatorMacedo, Leonardo L. P.
dc.creatorOliveira, Adeliana S.
dc.creatorMacedo, Francisco P.
dc.creatorAbreu, Luiz R. D.
dc.creatorSantos, Elizeu A.
dc.creatorSales, Mauricio P.
dc.date2010-09-30T12:18:39Z
dc.date2010-09-30T12:18:39Z
dc.date2006
dc.identifierSILVA, F. C. B. L.; ALCAZAR, A.; MACEDO, L. L. P.; OLIVEIRA, A. S.; MACEDO, F. P.; ABREU, L. R. D.; SANTOS, E. A.; SALES, M. P. (2006)
dc.identifier0965-1748
dc.identifierhttps://repositorio.ufrn.br/jspui/handle/1/3087
dc.descriptionSILVA, Fatima C. B. L. et al. Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets. Insect Biochemistry and Molecular Biology, v. 36, p. 561-569, 2006.ISSN: 0965-1748.DOI: 10.1016/j.ibmb.2006.04.004.
dc.descriptionThe digestive system of Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific a-galactosidasic and b-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of C. capitata was examined by bioassay. C. capitata was susceptible to SBTI which affected larval mass at ED50 3.01%
dc.languagepor
dc.publisherInsect Biochemistry and Molecular Biology
dc.rightsAcesso Aberto
dc.subjectCeratitis capitata
dc.subjectDisaccharidases
dc.subjectAcid proteinases
dc.subjectSerine proteinases
dc.subjectSBTI
dc.titleDigestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets
dc.typearticle


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