masterThesis
Propriedades bioativas dos polissacarídeos sulfatados da alga comestível Gracilaria birdiae
Fecha
2014-07-31Registro en:
OLIVEIRA, Letícia Castelo Branco Peroba de. Propriedades bioativas dos polissacarídeos sulfatados da alga comestível Gracilaria birdiae. 2014. 26f. Dissertação (Mestrado em Ciências da Saúde) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2014.
Autor
Oliveira, Letícia Castelo Branco Peroba de
Resumen
Red marine algae of the genus Gracilaria synthesize sulfated polysaccharides (PS) bioactive. But
many of these PS were not properly assessed, as is the case of PS synthesized by edible seaweed
Gracilaria birdiae. Previous studies showed that sulfated galactans this alga has anti-inflammatory
effect. In this work, a galactan (GB) of G. birdiae was obtained and evaluated by different tests. GB
showed anticoagulant activity in APTT assay. GB showed no toxicity to normal cells (3T3), but
inhibited the survival of cells of adenocarcinoma of the cervix (HeLa) and human pancreatic cancer
(Panc-1) 80% (1.5 mg / ml). GB was not able to hijack the OH radical or the superoxide radical.
However, showed activity electron donor in two different tests and presented iron chelator activity
(70% and 1.0 mg / ml) and Copper (70% at 0.5 mg / ml). The presence of a higher GB promotes
formation of crystals of calcium oxalate dihydrate small size, which is less aggressive, because GB
is able to interact with and stabilize the crystal that form. Furthermore, GB (2.0 mg / mL) was not
cytotoxic to human renal cells (HEK-293). The data lead us to propose that GB has a great potential
for the treatment of urolithiasis