IDENTIFICATION OF KURARINONE BY LC/MS AND INVESTIGATION OF ITS THERMAL STABILITY

dc.creatorLIU,PENGFEI
dc.creatorDENG,TIANSHENG
dc.creatorYE,CUIPING
dc.creatorQIN,ZHANGFENG
dc.creatorHOU,XIANGLIN
dc.creatorWANG,JIANGUO
dc.date2009-01-01
dc.date.accessioned2017-03-07T16:08:26Z
dc.date.available2017-03-07T16:08:26Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072009000100019
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/396782
dc.descriptionA herbal ingredient kurannone (7, 2', 4,-trihydroxy-8-lavandulyl-5-methoxy flavanone) was isolated from the roots of sophoraflavescens Ait by ultrasonic extraction, which is one of tradicional Chinese medicine (TCM) materials. A reliable high performance liquid chromatography with mass spectrometry (HPLC-MS) method was used for determination of kurarinone. C18 column (150x4.6 mm, 5 µm) and methanol-water mobile phase (70:30, by volume) were used in the chromatographic separation of active components from the herb. HPLC-ESI-MS (UV, 288 nm; m/z, 437.5 [M-H]-) was adopted for kurarinone identification. Kurarinone with purity of 95.5% was obtained by silica gel column foUowed by reverse-phase column chromatography. The thermal stability of kurarinone was investigated, which illustrates that kurarinone was stable below 25 °C in 48 h, but a degradation of 25.54% was observed at 80 °C for 2 h.
dc.formattext/html
dc.languageen
dc.publisherSociedad Chilena de Química
dc.sourceJournal of the Chilean Chemical Society v.54 n.1 2009
dc.subjectSophoraflavescens Ait
dc.subjectLeguminosae
dc.subjectFlavonoids
dc.subjectKurarinone
dc.subjectHPLC-ESI-MS
dc.subjectUltrasonic extraction
dc.subjectThermal stability
dc.titleIDENTIFICATION OF KURARINONE BY LC/MS AND INVESTIGATION OF ITS THERMAL STABILITY
dc.typeArtículos de revistas


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