article
Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4) after massive small bowel resection in rats
Fecha
2007Registro en:
1678-2674
Autor
Chacon, Dâmaso de Araújo
Araújo Filho, Irami
Villarim Neto, Arthur
Rêgo, Amália Cínthia Meneses
Azevedo, Ítalo Medeiros
Bernardo Filho, Mário
Brandão Neto, José
Medeiros, Aldo Cunha
Resumen
To evaluate the biodistribution of sodium pertecnetate (Na99mTcO4) in organs and tissues, the morphometry of
remnant intestinal mucosa and ponderal evolution in rats subjected to massive resection of the small intestine. Methods:
Twenty-one Wistar rats were randomly divided into three groups of 7 animals each. The short bowel (SB) group was
subjected to massive resection of the small intestine; the control group (C) rats were not operated on, and soft intestinal
handling was performed in sham rats. The animals were weighed weekly. On the 30th postoperative day, 0.l mL of
Na99mTcO4, with mean activity of 0.66 MBq was injected intravenously into the orbital plexus. After 30 minutes, the rats
were killed with an overdose of anesthetic, and fragments of the liver, spleen, pancreas, stomach, duodenum, small intestine,
thyroid, lung, heart, kidney, bladder, muscle, femur and brain were harvested. The biopsies were washed with 0.9%
NaCl.,The radioactivity was counted using Gama Counter WizardTM 1470, PerkinElmer. The percentage of radioactivity
per gram of tissue (%ATI-g) was calculated. Biopsies of the remaining jejunum were analysed by HE staining to obtain
mucosal thickness. Analysis of variance (ANOVA) and the Tukey test for multiple comparisons were used, considering
p<0.05 as signifi cant. Results: There were no signifi cant differences in %ATI-g of the Na99mTcO4 in the organs of the
groups studied (p>0.05). An increase in the weight of the SB rats was observed after the second postoperative week. The
jejunal mucosal thickness of the SB rats was signifi cantly greater than that of C and sham rats (p<0.05). Conclusion: In
rats with experimentally-produced short bowel syndrome, an adaptive response by the intestinal mucosa reduced weight
loss. The biodistribution of Na99mTcO4 was not affected by massive intestinal resection, suggesting that short bowel
syndrome is not the cause of misleading interpretation, if an examination using this radiopharmaceutical is indicated