Recuperação de proteína do soro de leite bovino usando cromatografia de interação hidrofóbica em leito expandido

Abstract

The present work deals with of cheese serum type “curdle” protein adsorption on the resin of hydrophobic interaction StreamlineTM Phenyl specially manufactured for expanded bed applications. A column of 2.6 cm of diameter for 30 cm of height, connected to a peristaltic pump was used. A sample of serum solution (250 mL) was applied to a column containing the resin StreamlineTM Phenyl (25 mL) previously equilibrated with in Tris/HCl (50 mM, pH 7.0) with NaCl (1.0 M). After washing step (300 ml of Tris/HCl 50 mM, pH 7.0 with NaCl (1.0M)) elution was carried out in buffer Tris/HCl (50 mM, pH 7.0) at room temperature with a fixed bed height of 5.0 cm. The results showed that it was possible to recover 37% of proteins initially contained in the serum just using one stage