doctoralThesis
Análise fitoquímica e avaliação da atividade antiescorpiônica e antiofídica do suco dos frutos de Hancornia speciosa Gomes (Apocynacea)
Fecha
2019-08-30Registro en:
YAMASHITA, Fabiana de Oliveira. Análise fitoquímica e avaliação da atividade antiescorpiônica e antiofídica do suco dos frutos de Hancornia speciosa Gomes (Apocynacea). 2019. 87f. Tese (Doutorado em Ciências Farmacêuticas) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2019.
Autor
Yamashita, Fabiana de Oliveira
Resumen
Accidents with venomous animals, mainly snakes and scorpions, are considered a
serious public health problem. In Brazil, ophidian accidents are mainly provoked by
snakes of the genus Bothrops, and those with scorpions, by the species Tityus
serrulatus. The conventional treatment is the use of antivenom serotherapy, which in
addition to presenting some limitations, such as difficult access in some regions and
high cost, is only able to neutralize the poison toxins, being inefficient in inhibiting the
endogenous reactions triggered by their presence. In this context, the aim of this paper
was to perform a phytochemical analysis of the fruit juice of Hancornia speciosa and
its ability to inhibit the effects of the venom of the scorpion Tityus serrulatus and the
snake Bothrops jararaca. The phytochemical analysis was performed using high
performance liquid chromatography MS/MS. The antiscorpionic activity of the juice was
evaluated using the animal model of acute pulmonary edema induced by the scorpion
venom T serrulatus. For this, Swiss mice received venom subcutaneously and were
treated orally with juice at doses of 100 and 200 mg / kg, using pre- and post-treatment
protocols. Two hours after venom administration, the animals were euthanized to
collect lungs, kidneys and blood. The following analyzes were performed with the
lungs: cytokine quantification, evans blue concentration, myeloperoxidase enzyme
(MPO) dosage and relative lung weight percentage; with the kidneys, nitrite dosage
and lipid peroxidation; and with blood, hematological and biochemical parameters were
evaluated. Regarding botropic venom, the ability of the juice to inhibit its local effects
was evaluated in the models of paw edema, hemorrhage, myotoxicity and
dermonecrosis. For this, Swiss mice of both sexes were used. In all models the juice
was administered orally at doses of 100 and 200 mg / kg and was administered shortly
after the botropic venom (post-treatment protocol). Through HPLC-DAD-MS / MS
analyzes 13 compounds were identified, the major ones identified as L-bornesitol,
quinic acid, chlorogenic acid and rutin. Observations regarding the inhibition of
antiscorpionic effects demonstrated that Hancornia speciosa juice was able to
significantly decrease some important parameters such as cytokine production,
myeloperoxidase activity, evans blue concentration in lung tissue, lipid peroxidation
and nitrite. Regarding the botropic effects, the juice was able to significantly reduce the
dermonecrotic lesion, the edematogenic activity, the hemorrhagic activity and the
concentrations of the creatine kinase and lactate dehydrogenase enzymes. From the
results, it can be concluded that Hancornia speciosa Gomes juice has great potential
to be used as adjuvant in the treatment of scorpion and botropic poisonings.