PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences

dc.creatorLian,Bin
dc.creatorZang,Jin-ping
dc.creatorHou,Wei-guo
dc.creatorYuan,Sheng
dc.creatorSmith,Donald L
dc.date2008-07-01
dc.date.accessioned2017-03-07T16:05:36Z
dc.date.available2017-03-07T16:05:36Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300011
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/395952
dc.descriptionIdentification of commercially important fungi, such as the valuable edible fungus Boletus edulis can be difficult considering visual or metabolic approaches. Based on phylogenetic analysis of the rDNA ITS sequence, a pair of specific primers was designed for differentiating B. edulis from other mushrooms by PCR. PCR was performed with total DNA as a template at an annealing temperature between 56-60ºC. Positive amplicons were obtained from B. edulis with all DNA templates from fruit bodies and cultured mycelium, but not from other fungal species at an annealing temperature of 60ºC. The result indicated that B. edulis could be clearly distinguished from other fungi by PCR, and there were no misidentifications under the reaction conditions used. The primers were also successfully employed to identify various tissues of B. edulis.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.sourceElectronic Journal of Biotechnology v.11 n.3 2008
dc.subjectBoletus edulis
dc.subjectdetection
dc.subjectedible fungi
dc.subjectinternal transcribed spacer
dc.subjectPCR
dc.subjectspecific primers
dc.titlePCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución