masterThesis
Imunoexpressão da Yes-Associated Protein (YAP) em hiperceratoses e displasias epiteliais orais
Fecha
2021-06-29Registro en:
COSTA, Lucas Melo da. Imunoexpressão da Yes-Associated Protein (YAP) em hiperceratoses e displasias epiteliais orais. 2021. 66f. Dissertação (Mestrado em Ciências Odontológicas) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2021.
Autor
Costa, Lucas Melo da
Resumen
The search for a biomarker that helps to predict the risk of malignant
transformation of oral potentially malignant disorders (OPMDs) represents a
great challenge, as it can help in the early and adequate management of patients.
This study evaluated the immunoexpression of Yes-Associated Protein (YAP) in
intraoral leukoplastic, erythroplastic or leukoerythroplastic lesions, with
histopathological diagnosis of hyperkeratosis or oral epithelial dysplasia (OED),
correlating this immunoexpression with the degree of morphological severity of
these lesions. The sample consisted of 20 cases of hyperkeratosis and 53 cases
of OED, in addition to 10 cases of normal oral mucosa (control group). To assess
the degree of dysplasia, the WHO grading (EL-NAGGAR et al., 2017) and the
Binary System (KUJAN et al., 2006) were used, and the immunohistochemical
profile of the YAP protein was evaluated through scores, which ranged from 0 to
3, based on their intracellular location (cytoplasmic or nuclear) and their
distribution in the epithelial tissue. For the analysis of the studied parameters,
Pearson's Chi-square and Fisher's Exact statistical tests were performed, in
addition to non-parametric tests (significance level of 95%). Mild dysplasias were
classified (100%) in the low risk of malignant transformation, while the moderate
ones were divided between low (47%) and high risk (53%), with severe
dysplasias, for the most part (71%), classified as high risk (p < 0.001). The control
group exhibited score 0 of immunostaining (80%), hyperkeratosis and mild
dysplasias (80% of booth) exhibit score 1, whereas in moderate (63%) and
severe dysplasia (79%), the predominant scores was 2 and 3; with a pattern of
nuclear immunostaining associated with the high risk of malignant transformation
suggested by the Binary System (p = 0.002). The immunoexpression of YAP was
similar between hyperkeratosis and mild dysplasias, which should attract greater
attention from professionals in cases of hyperkeratosis. Furthermore, the
expression of YAP appears to dichotomize OPMDs between lesions with low risk
of malignant transformation and lesions with high risk, which may suggest, in the
future, its use as a potential predictive marker of the progression for these lesions.