masterThesis
Estudo da influência do voo com o foguete VSB-30 no transcriptoma de plantas de Saccharum spp. (cana-de-açúcar)
Fecha
2017-05-26Registro en:
NASCIMENTO, Vladimir Vieira do. Estudo da influência do voo com o foguete VSB-30 no transcriptoma de plantas de Saccharum spp. (cana-de-açúcar). 2017. 65f. Dissertação (Mestrado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2017.
Autor
Nascimento, Vladimir Vieira do
Resumen
With the submission of sugarcane plants in a flight with the VSB-30 rocket,
histological and biochemical changes were observed in your different organs.
Therefore, we aimed to compare their transcriptome profiles to identify in tissue
samples from leaves and roots possible responses to the flight condition with the
VSB-30 rocket. For this, initially, 118 Differentially Expressed Transcripts were
detected with values of Log2FoldChange ≥2 or ≤-2 which were obtained by executing
the DESeq package, using the blind method. Through these comparisons that were
performed between the samples belonging to the groups Test flight with the VSB-30
rocket and Controls, it was verified that the highest sensitivities occurred in samples
of roots tissues, which allowed to select 77 specific transcripts. In addition, the
submission of the whole set of transcripts the tool STRING v.10. returned networks of
orthologous proteins from the organism Sorghum bicolor. However, the analyzes of
systems biology that were performed in the Cytoscape v.3.4 program, allowed to
identify for the roots samples changes in Genetics information Processing, with
induction, mainly, in biological processes of translation. In addition, the sample of
Vertical Rocket Root showed the highest sensitivity to the microgravity and
hypergravity conditions, which can be interpreted by biological systems as a
condition of abiotic stress, which results in induction of the biological processes that
involve the proteasome and repression for ribosome maturation. This, possibly, result
in progression of the cell cycle and provides maintenance of nitrogen levels in the
cell. In contrast, at tissue samples from leaves appeared as possible responses the
induction of the transcription. Furthermore, on the sample Vertical Rocket Leaf,
induction was detected on the metabolic pathway of glycine degradation and
decarboxylation. With all that, all these results indicate the main responses to the
flight with the VSB-30 rocket, which reaches microgravity and hypergravity
environments, which in plants are interpreted as a condition of environmental stress.