bachelorThesis
Efeito do extrato da cianobactéria Microcystis aeruginosa sobre o desenvolvimento larval do mosquito Aedes albopictus
Fecha
2021-09-15Registro en:
SILVA, Watson de Oliveira. Efeito do extrato da cianobactéria Microcystis aeruginosa sobre o desenvolvimento larval do mosquito Aedes albopictus. 2021. 36 f. Monografia (Graduação em Ecologia) – Universidade Federal do Rio Grande do Norte, Natal, 2021
Autor
Silva, Watson de Oliveira
Resumen
Dengue has become one of the main public health problems in the world due to the morbimortality of this viral disease with an epidemic potential. No less important, but with lower incidence rates Zika and Chikungunya are also viral diseases that affect millions of people worldwide for example. Like Dengue both arboviroses share something in common besides other aspects: vectorial transmission. In this sense, there is a need to develop alternative to the used insecticides which make it possible to stop the transmission of viral diseases without causing damage to the environment. In this work the effect of extracts obtained from the cultivation of a toxic cyanobacterium on the larval development of the Aedes albopictus mosquito was tested. The experiment was conducted under controlled laboratory conditions using an extract of a strain of the cyanobacterium Microcystis aeruginosa in which, because of its capacity to produce microcystis, has proven lethal to diferente aquatic organisms. For the execution of the experiment 360 larvae (L3) of A. albopictus were exposed to four treatments (concentrations: 25, 50, 75 and 100%) of the M. aeruginosa extract (strain LEA-04) (dilutions in WC medium), in addition to controls prepared with mineral water or WC medium. This experimental design was replicated in two sets: with and without addition of larval food (commercial feed used for fish farming). Both sets contained 18 experimental units (plastic containers - 50 mL), evenly subdivided into triplicates of the treatments and controls (volume of 30 mL each), totaling 36 experimental units. After incubation (10 larvae per experimental unit) monitoring for the presence of dead and dying larvae was done after 6, 24, 48, 72, and 96 hours of exposure. In the trial with the presence of food, only one larva died, in the control with WC medium. In addition approximately 83% of the larvae have turned into pupae. The others were already in the last stage of larval development (L4). In the test without the presence of food no larvae developed to the pupal stage, for at least 96 hours of observation. Houwever, there were no deaths in the entire monitoring. The results indicate that the strain extract was not effective in causing mosquito larval mortality. However, further experiments must continue to be conducted in order to seek alternative measures for the population control of vectors that transmit viral diseases.