masterThesis
Nanoemulsão estabilizada por hidrofobina, um carreador promissor para nutracêuticos
Fecha
2018-10-22Registro en:
OLIVEIRA, Christian Melo de. Nanoemulsão estabilizada por hidrofobina, um carreador promissor para nutracêuticos. 2018. 61f. Dissertação (Mestrado em Ciências da Saúde) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2018.
Autor
Oliveira, Christian Melo de
Resumen
Hydrophobin II (HFBII) is an amphiphilic biopolymer that could be explored to
stabilize oil-in-water nanoemulsions as nutraceutical delivery systems. This study
reports the production of HFBII-stabilized nanoemulsions by a spontaneous
emulsification process using copaiba oil as a bioactive lipid. HFBII was isolated from
a wild-type Trichoderma reesei and characterized. A 23
full factorial design with three
central points was used to obtain an optimal nanoemulsion system, whose physicalchemical properties were studied under different ionic strength and pH. The peptide
similarity search allowed the identification of a series of 6 ion fragments from the
isolated fraction, which can be attributed to the amino acid sequences of the HFBII
database. The optimal nanoemulsion system presented a nanoscale droplet size
(<200 nm), a narrow size distribution (PDI <0.2) and a zeta potential of ≈ -30 mV,
which was stable at low salt content and pH values close to the neutrality. These
results demonstrated the feasibility of using HFBII as a biopolymer to stabilize
nanoemulsion systems. Furthermore, the HFBII-stabilized nanoemulsion is a
promising carrier for nutraceuticals in food technology applications.