dc.contributorSilva Júnior, Arnóbio Antonio da
dc.contributor
dc.contributorhttp://lattes.cnpq.br/5028291382802042
dc.contributor
dc.contributorhttp://lattes.cnpq.br/2593509584288129
dc.contributorMorais, Waldenice de Alencar
dc.contributor
dc.contributorhttp://lattes.cnpq.br/2575667613995470
dc.contributorGomes, Ana Paula Barreto
dc.contributor
dc.contributorhttp://lattes.cnpq.br/1689823596741892
dc.contributorReynaud, Franceline
dc.contributor
dc.contributorhttp://lattes.cnpq.br/2561590161798118
dc.creatorFonsêca, Gabriela Diniz
dc.date.accessioned2016-04-27T23:04:29Z
dc.date.accessioned2022-10-05T23:11:13Z
dc.date.available2016-04-27T23:04:29Z
dc.date.available2022-10-05T23:11:13Z
dc.date.created2016-04-27T23:04:29Z
dc.date.issued2015-03-31
dc.identifierFONSÊCA, Gabriela Diniz. Micropartículas contendo nanopartículas de quitosana usando sulfato e genipina para liberação modificada da triancinolona: obtenção, caracterização e estudo em células tumorais. 2015. 80f. Dissertação (Mestrado em Ciências Farmacêuticas) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2015.
dc.identifierhttps://repositorio.ufrn.br/jspui/handle/123456789/20305
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3948861
dc.description.abstractChitosan is a polymer biocompatibility and biodegradability widely used in drug delivery systems. The co-crosslinking of chitosan with sodium sulfate and genipin, to form particulate systems is related of making them more resistant to acidic pH and to modulate the release kinetics for the oral route. Triamcinolone is a glucocorticoid with anti-inflammatory and immunosuppressive actions. The nanoparticles were prepared by co-crosslinking and characterized for particle size, PDI, zeta potential, crosslinking degree, encapsulation rate, morphology, infrared spectroscopy, thermal analysis, release kinetics and cells studies. The nanoparticles were prepared initially without genipin with sodium sulphate and the particles parameters were monitored in function of different ratio of drug / polymer, different concentrations of sodium sulfate and polysorbate 80 and the drip mode of crosslinkers on polymers. After optimizing conditions, the chosen system parameters without genipin included mean diameter of 312.20 ± 5.70 nm, PDI 0.342 ± 0.013 and zeta potential of 20.18 ± 2.28 mV. The genipin was introduced into the system analyzing different concentrations (0.5, 1.0 and 2.0 mM) and crosslinking times (3, 6, 12 and 24 h). Evaluating crosslinking time with genipin (0.5 mM) it was showed that varying the genipin reaction time the systems size ranged from 235.1 to 334.4 nm, the PDI from 0.321 to 0.392 and zeta potential 20.92 to 30.39 mV. The crosslinking degree that coud vary from 14 to 30 %. Nanoparticles without genipina, 6 h and 24 h crosslinking time were dried by spray-drying method. Analysis by scanning electron micrograph (SEM) revealed that the microparticles showed spherical morphology. The encapsulation rate was 75 ± 2.3 % using validated HPLC methodology. The infrared analysis showed chemical interactions between the components of the formulation. Thermal analysis showed that systems with a higher degree of crosslinking had a higher thermal stability. On release kinetics, increasing the degree of crosslinking was able to decrease the concentration and rate of release of triamcinolone. In studies with liver cancer cells (HepG2) and colon (HT-29), the microparticulate prepared with triamcinolone and 24 h of crosslinking with genipin showed a potential for antitumor activity in hepatic cell line HepG2. Therefore, a new delivery system for triamcinolone on polymeric nanoparticles of chitosan cocrosslinked with genipin and sodium sulfate was obtained with hepatic antitumor potential.
dc.languagepor
dc.publisherUniversidade Federal do Rio Grande do Norte
dc.publisherBrasil
dc.publisherUFRN
dc.publisherPROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACEUTICAS
dc.rightsAcesso Aberto
dc.subjectSistemas particulados
dc.subjectQuitosana
dc.subjectGenipina
dc.subjectTriancinolona
dc.subjectAntitumoral
dc.titleMicropartículas contendo nanopartículas de quitosana usando sulfato e genipina para liberação modificada da triancinolona: obtenção, caracterização e estudo em células tumorais
dc.typemasterThesis


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