Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans

Abstract

Escherichia coli and Candida albicans are responsible for harming both human and animal health. The most used method for the treatment of these diseases is the use of antibiotics, but the excessive use causes resistance of pathogenic microorganisms. The use of pyroligneous extract has been a widely used alternative. In order to facilitate the transport, handling and application, the use of a new formulation for the EP, in gel and solid, is possible from the addition of maltodextrin to the solution, in order to soften the odor and provide the viscosity range for the new products. formulas. The behavior and bactericidal and fungicidal potential of pyroligneous extract in gel and solid on Escherichia coli and Candida albicans were aimed at, in addition to establishing the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) and Minimum Fungicide Concentration (MFC). To establish the concentrations to be used in the microdilution test, the well disk agar diffusion test was performed in advance. For the microdilution test the concentrations used were: For E. coli with the EP in gel it was used: 15%, 5%, 1%, 0.5%, 0.25% and 0.125%; and for solid EP: 60, 50, 40, 30, 20, 18, 16, 10 and 1 mg. For C. albicans with the EP in gel was used: 4%, 3%, 2%, 1%, 0.5% and 0.25%; and for solid EP: 18, 16, 14, 12, 10, 8, 6, 4 mg. The concentrations were diluted and the microdilution test was mounted on two plates, one for the bacteria and one for the fungus. The concentrations were added, followed by their respective pathogens. The positive control treatment was performed with the addition of the culture medium corresponding to each one of them and the pathogen, while the negative control was used with the antibiotics gentamicin and fluconazole. After the incubation time, the Plates were analyzed in the Microplate Reader and the results submitted to one-way ANOVA, followed by the Tukey Test. The EP in gel on E. coli, obtained the same results for MIC and MBC: 1 ml, value that was the lowest non-significant concentration compared to the other concentrations. The MIC and MBC of solid EP for E. coli were 10 mg/m and 16 mg/ml, respectively. For the EP in gel on C. albicans, the MIC and MFC obtained equal values, 1 ml. For solid EP, MIC and MFC also showed the same results: 18 mg/ml for both. Therefore, it was possible to prove the antibacterial and antifungal action of the pyroligneous extract in gel and solid, in the respective concentrations, on Escherichia Coli and Candida albicans.