masterThesis
Caracterização físico-química e ações farmacológicas hepatoprotetora, antiinflamatória, pró-angiogênica, antioxidante e anticoagulante da fração rica em fucana 0,8FRF 0,8 da alga marrom Lobophora variegata
Fecha
2012-12-21Registro en:
WILL, Luiza Sheyla Evenni Porfírio. Caracterização físico-química e ações farmacológicas hepatoprotetora, antiinflamatória, pró-angiogênica, antioxidante e anticoagulante da fração rica em fucana 0,8FRF 0,8 da alga marrom Lobophora variegata. 2012. 109 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2012.
Autor
Will, Luiza Sheyla Evenni Porfírio
Resumen
This study examines the physical and chemical composition and the pharmacological
effects of brown seaweed FRF 0.8 Lobophora variegata. Fractionation of the crude
extract was done with the concentration of 0.8 volumes of acetone, obtaining the
FRF 0.8. The physicochemical characterization showed that it was a fucana sulfated.
Anti-inflammatory activity was assessed by paw edema model by the high rates of
inhibition of the edema and the best results were in the fourth hour after induction
(100 ± 1.4% at the dose of 75 mg / kg) and by the strong inhibitory activity of the
enzyme myeloperoxidase (91.45% at the dose of 25 mg / kg). The hepataproteção
was demonstrated by measurements of enzymatic and metabolic parameters
indicative of liver damage, such as bilirubin (reduction in 68.81%, 70.68% and
68.21% for bilirubin total, direct and indirect, respectively at a dose of 75 mg / kg),
ALT, AST and γ-GT (decrease of 76.93%, 44.58% and 50% respectively at a dose of
75 mg / kg) by analysis of histological slides of liver tissue, confirming that
hepatoprotective effect the polymers of carbohydrates, showing a reduction in tissue
damage caused by CCl4 and the inhibition of the enzyme complex of cytochrome P
450 (increasing sleep time in 54.6% and reducing the latency time in 71.43%). The
effectiveness of the FRF 0.8 angiogenesis was examined in chorioallantoic
membrane (CAM) of fertilized eggs, with the density of capillaries evaluated and
scored, showing an effect proangigênico at all concentrations tested FRF (10 mg-
1000 mg). The FRF showed antioxidant activity on free radicals (by inhibiting
Superoxide Radical in 55.62 ± 2.10%, Lipid Peroxidation in 100.15 ± 0.01%, Hydroxyl
Radical in 41.84 ± 0.001% and 71.47 Peroxide in ± 2.69% at concentration of 0.62
mg / mL). The anticoagulant activity was observed with prolongation of activated
partial thromboplastin time (aPTT) at 50 mg (> 240 s), showing that its action occurs
in the intrinsic pathway of the coagulation cascade. Thus, our results indicate that
these sulfated polysaccharides are an important pharmacological target