Artigo
A PCR/RFLP methodology to identify non-Amazonian Brazilian deer species
Date
2013-09-01Registration in:
Conservation Genetics Resources, v. 5, n. 3, p. 639-641, 2013.
1877-7252
1877-7260
10.1007/s12686-013-9870-3
WOS:000322619900010
2-s2.0-84880999606
Author
Universidade Estadual Paulista (Unesp)
Abstract
Due to the necessity of using noninvasive samples to study animals as elusive as the deer that occur in Brazil, we realized it was important to develop a PCR/RFLP protocol to assist in identifying such samples. Thus we developed a protocol in which a fragment of the cytochrome b gene is digested with two enzymes: SspI, which distinguishes species of the genus Mazama from Blastocerus dichotomus and Ozotoceros bezoarticus, and TAQα1 which permits differentiation between the species B. dichotomus and O. bezoarticus. © 2013 Springer Science+Business Media Dordrecht.