dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Instituto Agronômico (IAC) | |
dc.date.accessioned | 2014-05-27T11:29:34Z | |
dc.date.accessioned | 2022-10-05T18:51:10Z | |
dc.date.available | 2014-05-27T11:29:34Z | |
dc.date.available | 2022-10-05T18:51:10Z | |
dc.date.created | 2014-05-27T11:29:34Z | |
dc.date.issued | 2013-06-01 | |
dc.identifier | Annals of Microbiology, v. 63, n. 2, p. 533-540, 2013. | |
dc.identifier | 1590-4261 | |
dc.identifier | 1869-2044 | |
dc.identifier | http://hdl.handle.net/11449/75496 | |
dc.identifier | 10.1007/s13213-012-0500-1 | |
dc.identifier | WOS:000319074600014 | |
dc.identifier | 2-s2.0-84878020434 | |
dc.identifier | 4638952263502744 | |
dc.identifier | 0000-0001-9378-9036 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3924431 | |
dc.description.abstract | Lipase production by Trichoderma harzianum was evaluated in submerged fermentation (SF) and solid-state fermentation (SSF) using a variety of agro-industrial residues. Cultures in SF showed the highest activity (1.4 U/mL) in medium containing 0.5 % (w/v) yeast extract, 1 % (v/v) olive oil and 2.5 C:N ratio. This paper is the first to report lipase production by T. harzianum in SSF. A 1:2 mixture of castor oil cake and sugarcane bagasse supplemented with 1 % (v/w) olive oil showed the best results among the cultures in SSF (4 U/g ds). Lipolytic activity was stable in a slightly acidic to neutral pH, maintaining 50 % activity after 30 min at 50 C. Eighty percent of the activity remained after 1 h in 25 % (v/v) methanol, ethanol, isopropanol or acetone. Activity was observed with vegetable oils (olive, soybean, corn and sunflower) and long-chain triacylglycerols (triolein), confirming the presence of a true lipase. The results of this study are promising because they demonstrate an enzyme with interesting properties for application in catalysis produced by fermentation at low cost. © 2012 Springer-Verlag and the University of Milan. | |
dc.language | eng | |
dc.relation | Annals of Microbiology | |
dc.relation | 1.407 | |
dc.relation | 0,479 | |
dc.rights | Acesso restrito | |
dc.source | Scopus | |
dc.subject | Cassava cake | |
dc.subject | Castor bean | |
dc.subject | Corn husk | |
dc.subject | Lipase | |
dc.subject | Solid-state and submerged fermentation | |
dc.subject | Sugarcane bagasse | |
dc.subject | Trichoderma harzianum | |
dc.subject | Glycine max | |
dc.subject | Helianthus | |
dc.subject | Hypocrea lixii | |
dc.subject | Manihot esculenta | |
dc.subject | Ricinus communis | |
dc.subject | Zea mays | |
dc.title | Comparing submerged and solid-state fermentation of agro-industrial residues for the production and characterization of lipase by Trichoderma harzianum | |
dc.type | Artigo | |