Trabalho apresentado em evento
Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluans on solid state fermentation
Fecha
2007-04-01Registro en:
Applied Biochemistry and Biotechnology, v. 137-140, n. 1-12, p. 281-288, 2007.
0273-2289
10.1007/s12010-007-9058-y
WOS:000246616900026
2-s2.0-52949136274
7091241742851920
9424175688206545
Autor
Universidade Estadual Paulista (Unesp)
Resumen
This article investigates a strain of the yeast Aureobasidium pullulans for cellulase and hemicellulase production in solid state fermentation. Among the substrates analyzed, the wheat bran culture presented the highest enzymatic production (1.05 U/mL endoglucanase, 1.3 U/mL β-glucosidase, and 5.0 U/mL xylanase). Avicelase activity was not detected. The optimum pH and temperature for xylanase, endoglucanase and β-glucosidase were 5.0 and 50, 4.5 and 60, 4.0 and 75°C, respectively. These enzymes remained stable between a wide range of pH. The β-glucosidase was the most thermostable enzyme, remaining 100% active when incubated at 75°C for 1 h. © 2007 Humana Press Inc.
Materias
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