Artigo
Inhibition of eukaryotic translation initiation factor 5A (eIF5A) hypusination impairs melanoma growth
Fecha
2007-01-01Registro en:
Cell Biochemistry and Function, v. 25, n. 1, p. 109-114, 2007.
0263-6484
1099-0844
10.1002/cbf.1351
WOS:000243581300012
2-s2.0-33846292223
Autor
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Resumen
The eukaryotic translation initiation factor 5A (eIF5A) undergoes a specific post-translational modification called hypusination. This modification is required for the functionality of this protein. The compound N1-guanyl-1,7-diaminoheptane (GC7) is a potent and selective inhibitor of deoxyhypusine synthase, which catalyses the first step of eIF5A hypusination process. In the present study, the effects of GC7 on cell death were investigated using two cell lines: melan-a murine melanocytes and Tm5 marine melanoma. In vitro treatment with GC7 increased by 3-fold the number of cells presenting DNA fragmentation in Tm5 cells. Exposure to GC7 also decreased viability to both cell lines. This study also describes, for the first time, the in vivo antitumour effect of GC7, as indicated by impaired melanoma growth in C57BL/6 mice. Copyright © 2006 John Wiley & Sons, Ltd.