Artigo
Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide
Fecha
2006-05-01Registro en:
Journal of Biological Sciences, v. 6, n. 3, p. 596-603, 2006.
1727-3048
1812-5719
10.3923/jbs.2006.596.603
2-s2.0-33745769561
Autor
Universidade de Taubaté (UNITAU)
Centro Universitário de Araraquara (UNIARA)
Universidade Estadual Paulista (Unesp)
Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP)
Universidade Federal de São Carlos (UFSCar)
Resumen
The aim of this study was to examine the role of nifedipine and Nitric Oxide (NO) on salivary flow and compounds (salivary amylase, saliva total proteins, saliva calcium, sodium and potassium). Male Holtzman rats weighting 200-250 g were anesthetized with zoletil 50 mg kg -1 (tiletamine chloridrate 125.0 mg and zolazepan chloridrate 125.0 mg) into quadriceps muscle and stainless steel cannulas were implanted into their lateral ventricle of the brain (LV). Animals in divided group were injected with nifedipine (50 μg μL -1) alone and in combination with 7-nitroindazol (7-NIT) (40 μg μL -1), neuronal NO Sinthase Inhibitor (nNOSI) and Sodium Nitroprussate (SNP) (30 μg μL -1) NO donor agent. As a secretory stimuli, pilocarpine dissolved in isotonic was administered intraperitoneally (ip) at a dosage of 10 mg kg -1 body weight. Saliva was collected for 7 min with four cotton balls weighing approximately 20 mg each, two of which were placed on either side of the oral cavity, with the other two placed under the tongue. Nifedipine treatment induced a reduction in saliva secretion rate and concentration of amylase, total protein and calcium without changes in sodium and potassium concentration in comparison with controls. Co-treatment of animals with nifedipine and SNP retained flow rate and concentration of amylase, total protein and calcium in normal levels. Co-treatment of animals with nifedipine and 7-NIT potentiated the effect of nifedipine on the reduction of saliva secretion and concentrations of amylase, total protein and calcium. Nifedipine (dihydroperidine) calcium-channel blocker widely in use is associated with salivary dysfunction acting in the central nervous system structures. NO might be the mechanism for protective effect against the nifedipine-induce salivary dysfunction, acting in the CNS. © 2006 Asian Network for Scientific Information.
Materias
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Arginase-endothelial nitric oxide synthase imbalance contributes to endothelial dysfunction during chronic intermittent hypoxia
Krause B.J.; Del Rio R.; Moya E.A.; Marquez-Gutierrez M.; Casanello P.; Iturriaga R. (Lippincott Williams and Wilkins, 2015) -
Diet-induced obesity impairs AKT signalling in the retina and causes retinal degeneration
Marçal, Anderson C.; Leonelli, Mauro; Fiamoncini, Jarlei; Deschamps, Francisco C.; Rodrigues, Maria A.M.; Curi, Rui; Carpinelli, Angelo R.; Britto, Luiz R.G.; Carvalho, Carla R.O. -
Diet-induced obesity impairs AKT signalling in the retina and causes retinal degeneration
Universidade de São Paulo (USP); Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (EPAGRI); Universidade Estadual Paulista (Unesp) (2013-01-01)Retinopathy, a common complication of diabetes, is characterized by an unbalanced production of nitric oxide (NO), a process regulated by nitric oxide synthase (NOS). We hypothesized that retinopathy might stem from changes ...