Artigo
Eucalyptus ESTs involved in the production of 9-cis epoxycarotenoid dioxygenase, a regulatory enzyme of abscisic acid production
Fecha
2005-12-01Registro en:
Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 640-643, 2005.
1415-4757
1678-4685
10.1590/S1415-47572005000400020
S1415-47572005000400020
WOS:000204389800020
2-s2.0-30844450109
2-s2.0-30844450109.pdf
8649222099176162
9855493448161702
3845989485833395
0165348738208319
0000-0003-0431-5942
0000-0002-6924-835X
0000-0003-4524-954X
Autor
Universidade Estadual Paulista (Unesp)
Resumen
Abscisic acid (ABA) regulates stress responses in plants, and genomic tools can help us to understand the mechanisms involved in that process. FAPESP, a Brazilian research foundation, in association with four private forestry companies, has established the FORESTs database (https://forests.esalq.usp.br). A search was carried out in the Eucalyptus expressed sequence tag database to find ESTs involved with 9-cis epoxycarotenoid dioxygenase (NCED), the regulatory enzyme for ABA biosynthesis, using the basic local BLAST alignment tool. We found four clusters (EGEZLV2206B11.g, EGJMWD2252H08.g, EGBFRT3107F10.g, and EGEQFB1200H10.g), which represent similar sequences of the gene that produces NCED. Data showed that the EGBFRT3107F10.g cluster was similar to the maize (Zea mays) NCED enzyme, while EGEZLV2206B11.g and EGJMWD2252H08.g clusters were similar to the avocado (Persea americana) NCED enzyme. All Eucalyptus clusters were expressed in several tissues, especially in flower buds, where ABA has a special participation during the floral development process. Copyright by the Brazilian Society of Genetics.