Artigo
rDNA-RFLP identification of Candida species in immunocompromised and seriously diseased patients
Fecha
2004-07-01Registro en:
Canadian Journal of Microbiology, v. 50, n. 7, p. 514-520, 2004.
0008-4166
10.1139/w04-025
WOS:000224057700009
2-s2.0-6344275553
6543563161403421
0000-0002-2416-2173
Autor
Universidade Federal de Minas Gerais (UFMG)
FIOCRUZ
Universidade Estadual Paulista (Unesp)
Resumen
PCR was used to amplify a targeted region of the ribosomal DNA of 76 Candida spp. isolates from immunocompromised and seriously diseased patients. Thirty-seven strains isolated from different anatomical sites of 11 patients infected with HIV (Vitória, ES, Brazil), 26 isolates from patients under treatment at Odilon Behrens Hospital and 13 isolates from skin and urine samples from São Marcos Clinical Analysis Laboratory (Belo Horizonte, Brazil) were scored. Fragments of rDNA were amplified using primer pairs ITS1-ITS4, for the amplification of ITS1 and ITS2 regions, including the gene for the 5.8s subunit. Amplification resulted in fragments ranging in size from 350 to 950 bp. Amplicons were digested with eight restriction enzymes. A pattern of species-specificity among the different medically important Candida species could be identified following restriction digestion of the PCR products. Candida albicans was the species most frequently observed, except for the group of newborns under treatment at the Odilon Behrens Hospital and for the isolates from the clinical analysis laboratory. C. parapsilosis was the species most frequently observed in these two groups.
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