Preparation of N2, N2,7-trimethylguanosine affinity columns

dc.contributorCSIC
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorHospitalet de Llobregat
dc.contributorEMBL
dc.date.accessioned2014-05-27T11:19:41Z
dc.date.accessioned2022-10-05T17:38:17Z
dc.date.available2014-05-27T11:19:41Z
dc.date.available2022-10-05T17:38:17Z
dc.date.created2014-05-27T11:19:41Z
dc.date.issued1999-01-01
dc.identifierNucleosides and Nucleotides, v. 18, n. 1, p. 125-136, 1999.
dc.identifier0732-8311
dc.identifierhttp://hdl.handle.net/11449/65686
dc.identifier10.1080/07328319908045599
dc.identifier2-s2.0-0032589080
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3915583
dc.description.abstract2,2,7-trimethylguanosine (TMG) binding proteins from human cells were purified through TMG-affinity columns. TMG synthesis was improved and the TMG obtained was shown to be similar to the TMG in the 5' cap of the UsnRNAs. The eluates obtained with TMG-affinity chromatographies were very different from those isolated with m7G-affinity columns, thus suggesting that specific TMG- binding proteins were obtained. The fraction may be enriched with factors associated with import and/or hypermethylation of UsnRNPs.
dc.languageeng
dc.relationNucleosides and Nucleotides
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectbinding protein
dc.subjectguanosine derivative
dc.subjectsmall nuclear RNA
dc.subjectantibody
dc.subjectcarrier protein
dc.subjectdrug derivative
dc.subjectfungal protein
dc.subjectguanosine
dc.subjecthemocyanin
dc.subjectkeyhole limpet hemocyanin
dc.subjectkeyhole-limpet hemocyanin
dc.subjectmonoclonal antibody
dc.subjectN(2),N(2),7 trimethylguanosine
dc.subjectN(2),N(2),7-trimethylguanosine
dc.subjectnuclear protein
dc.subjectsepharose
dc.subjectserum albumin
dc.subjectaffinity chromatography
dc.subjectanimal experiment
dc.subjectextraction
dc.subjecthuman
dc.subjectmouse
dc.subjectnonhuman
dc.subjectnucleotide metabolism
dc.subjectprotein methylation
dc.subjectprotein purification
dc.subjectspliceosome
dc.subjectsynthesis
dc.subjectanimal
dc.subjectcell nucleus
dc.subjectchemistry
dc.subjectcytoplasm
dc.subjectHeLa cell
dc.subjectinstrumentation
dc.subjectisolation and purification
dc.subjectmethodology
dc.subjectrabbit
dc.subjectSaccharomyces cerevisiae
dc.subjectAnimals
dc.subjectAntibodies
dc.subjectAntibodies, Monoclonal
dc.subjectCarrier Proteins
dc.subjectCell Nucleus
dc.subjectChromatography, Affinity
dc.subjectCytoplasm
dc.subjectFungal Proteins
dc.subjectGuanosine
dc.subjectHela Cells
dc.subjectHemocyanin
dc.subjectHumans
dc.subjectMice
dc.subjectNuclear Proteins
dc.subjectRabbits
dc.subjectSepharose
dc.subjectSerum Albumin
dc.titlePreparation of N2, N2,7-trimethylguanosine affinity columns
dc.typeArtigo


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