Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis

Artigo

Date

1996-10-19

Registration in:

Journal of Medical and Veterinary Mycology, v. 34, n. 4, p. 273-277, 1996.

0268-1218

1369-3786

1460-2709

http://hdl.handle.net/11449/64865

10.1080/02681219680000461

WOS:A1996VC30200007

2-s2.0-0029792395

2-s2.0-79961158106

0053567153623569

http://repositorioslatinoamericanos.uchile.cl/handle/2250/3914847

Author

Universidade Estadual Paulista (Unesp)

Universidade Federal de São Paulo (UNIFESP)

Institutions

Universidade Paulista Júlio de Mesquita Filho (Brasil)

Abstract

Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl S300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.

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