Artigo
Myenteric Denervation Downregulates Galectin-1 and-3 Expression in Gastric Carcinogenesis
Fecha
2011-06-01Registro en:
Digestive Diseases and Sciences. Dordrecht: Springer, v. 56, n. 6, p. 1637-1644, 2011.
0163-2116
10.1007/s10620-010-1516-7
WOS:000290770900009
Autor
Universidade Federal de São Paulo (UNIFESP)
Faculdade de Medicina de São José do Rio Preto (FAMERP)
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Resumen
Background This study evaluated the galectin-1 and -3 expression during N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis in denervated rat stomachs using benzalkonium chloride.Method Four experimental situations were evaluated: nondenervated and denervated stomachs without lesions and nondenervated and denervated stomachs with lesions. Sections of the pyloric region were stained with toluidine blue and incubated with mouse monoclonal anti-Gal-1 and rabbit polyclonal anti-Gal-3 for histopathological and immunohistochemical analysis, respectively.Result MNNG caused the development of benign and malignant epithelial lesions, which were more pronounced in nondenervated stomachs with lesions and accompanied by inflammatory cell-enriched stroma. By immunostaining, the epithelial cells, blood vessels, muscle layer, and myenteric plexus were Gal-1 and -3 positive. Gal-3 was also detected in the gastric crypts, mucus secretion, and fibroblasts of pyloric fragments. Development of lesions in denervated stomachs was associated with a significant decrease in Gal-1 and -3 expression in epithelial cells, mast cells, and neutrophil cytoplasm, compared with that of nondenervated stomach lesions (P < 0.01; P < 0.001; P < 0.001, respectively).Conclusion These results demonstrate that myenteric denervation downregulates endogenous Gal-1 and -3 expression, which might inhibit tumor development in this experimental model.