Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia

dc.creatorWilson S,Myra
dc.creatorOtth L,Carola
dc.creatorMedina S,Gustavo
dc.creatorOtth R,Laura
dc.creatorFernández J,Heriberto
dc.creatorArce,María
dc.creatorZaror C,Angela
dc.creatorLizama,Víctor
dc.creatorGil D,Mónica
dc.creatorvon Chrismar,Ana María
dc.date2007-05-01
dc.date.accessioned2017-03-07T15:49:01Z
dc.date.available2017-03-07T15:49:01Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000500007
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/391298
dc.descriptionBackground: Methicillin resistant strains of Staphylococcus aureus (MRSA) are an important cause of nosocomial infections. Aim: To determine the genotypes of MRSA strains. Material and methods: Fifty five strains of MRSA, isolated from patients hospitalized in Hospital Base Valdivia, were studied. The phenotype was determined through MicroScan® in all strains and by minimum inhibitory concentration (MIC) in 41. The genotype of the strains was analyzed by a duplex polymerase chain reaction (PCR) of the mecA gene, amplifying eight hypervariable DNA regions associated to such gene. Results: According to MIC, 88% of strains had a pattern of resistance against multiple antimicrobial (penicillin, ampicillin, cephradine, gentamycin, ciprofloxacin, ¡incomycin and erythromycin). Vancomicin resistan strains were not detected. Only 53 strains (96%) had at least one of the eight hypervariable regions and were classified as MRSA. Genotypic patterns types 15 were the most commonly detected in 38% and 34% of strains, respectively. MicroScan® erroneously classified five strains in an incorrect phenotype, according to results obtained with duplex PCR. MIC results did not differ from those of duplex PCR. Conclusions: Duplex- PCR is a useful tool to detect hyper variable regions associated to mecA gene
dc.formattext/html
dc.languagees
dc.publisherSociedad Médica de Santiago
dc.sourceRevista médica de Chile v.135 n.5 2007
dc.subjectDrug resistance, bacterial
dc.subjectMethicillin
dc.subjectStaphylococcu aureus
dc.titleGenotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia
dc.typeArtículos de revistas


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