A Preliminary and Qualitative Metallomics Study of Mercury in the Muscle of Fish from Amazonas, Brazil

Artigo

Fecha

2012-12-01

Registro en:

Biological Trace Element Research. Totowa: Humana Press Inc, v. 150, n. 1-3, p. 195-199, 2012.

0163-4984

http://hdl.handle.net/11449/40765

10.1007/s12011-012-9502-x

WOS:000312075800028

http://repositorioslatinoamericanos.uchile.cl/handle/2250/3911720

Autor

Universidade Estadual Paulista (Unesp)

Universidade de Brasília (UnB)

Universidade Estadual de Campinas (UNICAMP)

Institución

Universidade Paulista Júlio de Mesquita Filho (Brasil)

Resumen

This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish species from Amazonas, Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis and subsequent evaluation of mercury by synchrotron radiation X-ray fluorescence. The fluorescence spectra revealed mercury in two protein spots. The mercury-containing protein spots showed molecular weights of 20.8 +/- 0.7 and 19.8 +/- 0.5 kDa and isoelectric points of 5.6 +/- 0.2 and 7.5 +/- 0.3, respectively.

Materias

Metallomics study

Mercury in fish

2D PAGE

SR XRF

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