Artigo
Aracatuba virus: A vaccinialike m virus associated with infection in humans and cattle
Fecha
2003-02-01Registro en:
Emerging Infectious Diseases. Atlanta: Center Disease Control, v. 9, n. 2, p. 155-160, 2003.
1080-6040
WOS:000181034600002
WOS000181034600002.pdf
2232625970712015
2976129874554698
Autor
Universidade Federal de Minas Gerais (UFMG)
NIH
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Resumen
We describe a vaccinialike virus, Aragatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, an DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleoticle sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Aragatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Aragatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.