Assay of Serum Glutamic-Oxaloacetic Transaminase Using Malate Dehydrogenase Preparation Obtained from Streptomyces aureofaciens

Artigo

Fecha

1995-01-01

Registro en:

Analytical Letters. New York: Marcel Dekker Inc., v. 28, n. 13, p. 2305-2316, 1995.

0003-2719

http://hdl.handle.net/11449/32395

10.1080/00032719508000374

WOS:A1995RV75900005

http://repositorioslatinoamericanos.uchile.cl/handle/2250/3904966

Autor

Universidade Estadual de Campinas (UNICAMP)

Universidade Estadual Paulista (Unesp)

Institución

Universidade Paulista Júlio de Mesquita Filho (Brasil)

Resumen

A modified spectrophotometric method for serum glutamic-oxaloacetic transaminase (SGOT) assay was developed. A crude cell-free extract from Streptomyces aureofaciens which showed a high level of malate dehydrogenase (MDH) activity (E.C. 1.1.1.37) was used as the enzymatic indicator. The lyophilized microbial preparation was used without previous purification and was quite stable under refrigeration for one year. Serum sample assays using both the method utilizing the crude cell extract and an enzymatic commercial kit showed good correlation.

Materias

TRANSAMINASE

SGOT

MALATE DEHYDROGENASE

STREPTOMYCES

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