Artigo
Determination of nitrite in meat products and water using dapsone with combined spot test/diffuse reflectance on filter paper
Fecha
2012-10-15Registro en:
Food Chemistry. Oxford: Elsevier B.V., v. 134, n. 4, p. 2546-2551, 2012.
0308-8146
10.1016/j.foodchem.2012.04.068
WOS:000305859800113
5978908591853524
3192125098361121
0000-0003-0197-7369
Autor
Universidade Estadual Paulista (Unesp)
Resumen
This paper describes a simple, portable and environmentally friendly procedure for the determination of nitrite by combined spot test/diffuse reflectance spectroscopy on filter-paper. The method is based on the diazotization reaction of dapsone (4,4'-diamino-diphenyl sulphone, DAP) and (naphthyl)ethylenediamine hydrochloride (NED) with nitrite in acidic medium, yielding a coloured compound on the surface of a filter paper. Experimental design methodologies were used to optimise the measurement conditions. The reflectometric measurements were carried out at 545 nm and the linear range obtained was 0.29-5.0 mg L-1 of nitrite (R = 0.997), with detection and quantification limits of 0.09 and 0.29 mg L-1, respectively. The proposed method was applied successfully to the analysis of nitrite in meat products and well water samples. (C) 2012 Elsevier Ltd. All rights reserved.