Improved affinity selection using phage display technology and off-rate based selection

dc.creatorYuan,Bin
dc.creatorSchulz,Philip
dc.creatorLiu,Ruitian
dc.creatorSierks,Michael R.
dc.date2006-04-01
dc.date.accessioned2017-03-07T15:43:36Z
dc.date.available2017-03-07T15:43:36Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200011
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/389880
dc.descriptionFlow systems such as a BIAcore biosensor can be very efficient tools to isolate high affinity antibody fragments from affinity matured phage display libraries. Here we show that using flow based selection, we can readily isolate a variant with a 35-fold higher affinity, especially with a 7 fold better off-rate, compared to the parent clone after only a single round of selection from a second generation affinity matured phage display library. The flow system represents a fast method to isolate affinity improved antibody fragments and can be particularly useful for isolating antibodies to antigens that have poor solubility, are toxic to the host cell, or prone to aggregation.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.sourceElectronic Journal of Biotechnology v.9 n.2 2006
dc.subjectaffinity maturation
dc.subjectbeta-amyloid (Abeta)
dc.subjectdissociation rate
dc.subjectphage display
dc.titleImproved affinity selection using phage display technology and off-rate based selection
dc.typeArtículos de revistas


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