Artigo
Microscopia de força atômica aplicada em imunoensaios
Fecha
2006-02-01Registro en:
Química Nova. Sociedade Brasileira de Química, v. 29, n. 1, p. 137-142, 2006.
0100-4042
10.1590/S0100-40422006000100024
S0100-40422006000100024
S0100-40422006000100024.pdf
1923726000036625
Autor
Universidade Estadual Paulista (Unesp)
Resumen
Affinity reactions have been used for specific detection of their complementary partners and an enormous variety of enzyme-linked immunosorbent assay (ELISA) formats are used in research and in routine serological tests. With the advent of the atomic force microscopy (AFM) technique, the immune reactions have been monitored by these devices. In the present article we focus on applications of AFM to immunoassays. After introducing the basic concepts of AFM, a brief discussion on the monitoring of the interactions between antigens and antibodies through both topographic image and biosensor systems is presented.